Determination of Specific Anti - Toxocara Antibodies in Patients with Cardiac Disease

Eleonora Kaneva, Rumen Harizanov, Stefka Krumova, Petya Genova-Kalu, Iskra Rainova, Nina Tsvetkova, Iskren Kaftandjiev, Mariya Pishmisheva

Abstract


Aim of this study is to find the seroprevalence of specific anti - Toxocara IgG antibodies among patients with inflammatory heart disease and to evaluate the significance of this parasite as a possible etiological agent of such pathology. We performed a serological study of 41 patients with heart disease (myocarditis, pericarditis and endocarditis) for presence of specific anti-Toxocara IgG antibodies. We used ELISA for primary screening, and Western blot as a confirmatory method. Presence of specific anti-Toxocara IgG antibodies in ELISA was detected in three (7.3%) of the serum samples, and another two (4.9%) were with borderline values. These serum samples were further examined in Western blot and three of them (7.3%) displayed disease-specific bands. We do not in any way claim that in these cases Toxocara infection is the cause of inflammatory heart disease, but the data from the study shows that such a link is possible.


Keywords


Toxocariasis, Heart Disease, Differential Diagnosis, Western Blot, ELISA

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References


Beaver P, Snyder C, Carrera G, Dent J, Lafferty J. Chronic eosinophilia due to visceral larva migrans: report of three cases. Pediatrics 1952;9:7-19.

Xinou E, Lefkopoulos A, Gelagoti M, et al. CT and MR imaging findings in cerebral toxocaral disease. Amer J Neuroradiol 2003;24(4): 714-718.

Page A, Hamilton A, Maizels R. Toxocara canis: monoclonal antibodies to carbohydrate epitopes of secreted (TES) antigens localize to different secretion-related structures in infective larvae. Exp Parasitol 1992;75(1): 56-71.

Despommier D. Toxocariasis: Clinical aspects, epidemiology, medical ecology and molecular aspects. Clin Microbiol Rev 2003;16(2):265-272.

Wilder HC. Nematode endophthalmitis. Trans Am Acad Ophthalmol Otolaryngol 1950;55: 99-109.

Taylor M, Keane C, O’Connor P, Mulvihill E. Holland C. The expanded spectrum of toxocaral disease. Lancet 1988;1(8587):692-5.

Glickman L, Schantz P, Cypess R. Epidemiological characteristics and clinical findings in patients with serologically proven Toxocariasis. Trans R Soc Trop Med Hyg 1979;73(3): 254-8.

Hartleb M, Januszewski K. Severe hepatic involvement in visceral larva migrans. Eur J Gastroenterol Hepatol 2001;13(10): 1245-1249.

Kuziemski K, Jassem E, Mierzejewska E. Lung manifestation of visceral larva migration syndrome due to Toxocara canis infection. Pneumonol Alergol Pol 1999;67(11-12): 554-557.

Magnaval JF, Galindo L, Glickman T, Clanet M. Human Toxocara infection of the central nervous system and neurological disorders: a case-cоntrol study. Parasitology 1997;115(Pt 5): 537-543.

Kuenzli E, Neumayr A, Chaney M, Blum J. Toxocariasis-associated cardiac diseases – A systematic review of the literature. Acta Tropica 2016;154: 107-120. doi: 10.1016/j.actatropica.2015.11.003.

Lee R, Moore L, Bottazzi M, Hotez P. Toxocariasis in North America: a systematic review. PLoS Negl Trop Dis 2014;8(8):e3116. doi: 10.1371/journal.pntd.0003116.

Dao A, Virmani R. Visceral larva migrans involving the myocardium: report of two cases and review of the literature. Pediatr Pathol 1986;6(4): 449-456.

Abe K, Shimokawa H, Kubota T, Nawa Y, Takeshita A. Myocarditis associated with visceral larva migrans due to Toxocara canis. Intern Med 2002;41(9): 706-708.

Schaffer S, Dimayuga E, Kayes S. Development and characterization of a model of eosinophil-mediated cardiomyopathy in rats infected with Toxocara canis. Am J Physiol 1992;262(5 Pt 2):H1428-34.

Woodruff A. Toxocariasis. Br Med J 1970;3(5724):663-9.

De Savigny D. In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. J Parasitol 1975;61(4): 781-782.

Magnaval JF, Fabre R, Maurières P, Charlet JP, de Larrard B. Application of the western blotting procedure for the immunodiagnosis of human toxocariasis. Parasitol Res 1991;77(8), 697-702.

Baldisserotto M, Conchin C, Da Soares M, Araujo M, Kramer B. Ultrasound findings in children with toxocariasis: report on 18 cases. Pediatr Radiol 1999;29(5): 316–319.

Dupas B, Barrier J, Barre P. Detection of Toxocara by computed tomography. Br J Radiol 1986;59(701): 518–519.

Ivanova S, Angelova S, Stoyanova A, et al. Serological and molecular biological studies of Parvovirus B19, Coxsackie B Viruses, and Adenoviruses as a potential cardiotropic viruses in Bulgaria. Folia Medica 2016;58 (4): 250-255. doi:10.1515/folmed-2016-0036

Genova-Kalou P, Ivanova S, Pavlova A, Trendafilova E, Pencheva D. Seroepidemiological and molecular biology study of Coxiella Burnetii in laboratory samples of patients with cardiac complications. Sixteenth national congress of Clinical Microbiology and Infections of Bulgarian Association of Microbiologists. Sofia, May 10-12, 2018. Park-hotel „Moskva”. Abstract book, pp.39-40.

Drory Y, Turetz Y, Hiss Y, et al. Sudden unexpected death in persons less than 40 years of age. Am J Cardiol 1991;68 (13): 1388-92.

Bowles N, Vallejo J. Viral causes of cardiac inflammation. Curr Opin Cardiol 2003;18 (3): 182-8.

Shah S, McGowan J. Rickettsial, ehrlichial and Bartonella infections of the myocardium and pericardium. Front Biosci 2003;8: e197-201.

Wasi F, Shuter J. Primary bacterial infection of the myocardium. Front Biosci 2003;8: s228-231.

Hidron A, Vogenthaler N, Santos-Presiado J, Rodriguez-Morales A, Franco-Paredes C, Rassi A Jr. Cardiac involvement with parasitic infections. Clin Microbiol Rev 2010;23(2):324-49. doi: 10.1128/CMR.00054-09.

Kim J, Chung W, Chang K, et al. Eosinophilic myocarditis associated with visceral larva migrans caused by Toxocara canis infection. J. Cardiovasc. Ultrasound 2012;20(3):150-3. doi: 10.4250/jcu.2012.20.3.150.

Becroft D. Infection by the dog roundworm Toxocara canis and fatal myocarditis. N Z Med J 1964;63: 729-732.

Vargo T, Singer D, Gillette P, Fernbach D. Myocarditis due to visceral larva migrans. J Pediatr 1977;90(2): 322-323.

Kaneva E, Rainova I, Harizanov R, Nikolov G, Kaftandjiev I, Mineva I. Study of Toxocara seroprevalence among patients with allergy and healthy individuals in Bulgaria. Parasite Immunol 2015;37(10): 505-509. doi: 10.1111/pim.12217.

Cypess R, Karol M, Zidian J, Glickman L, Gitlin D. Larva-specific antibodies in patients with visceral larva migrans. J Infect Dis 1977;135(4):633–640.

Lynch N, Wilkes L, Hodgen A, Turner K. Specificiy of Toxocara ELISA in tropical populations. Parasite Immunol 1988;10: 323-337.




DOI: http://dx.doi.org/10.7575/aiac.abcmed.v.9n.3p.36

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