In vitro evaluation of antibacterial effect of combination of honey and Aloe vera extract against Enterococcus faecalis and Escherichia coli

Paria Motahari, Hossein Samadi Kafil, Zahra Taheri Bonab

Abstract


Antimicrobial agents are used as intra-canal drugs or canal cleaners for complete dental root canal cleaning. This new study attempts to compare the antibacterial effect of 2% chlorhexidine and combination of honey and Aloe vera extract (H-Av mixture) against Enterococcus faecalis (E.faecalis) and Escherichia coli (E.coli) which are the most important pathogens isolated from infectious root canals. Enterococcus faecalis (ATCC® 29212™) and Escherichia coli (ATCC® 25922™) were cultured in the tryptic soy broth medium. Dilution of H-Av mixture by the method of macrodilution to determine Minimum Inhibitory Concentration was investigated. In this paper agar well diffusion and colony count methods were also used to ensure the accuracy of the results. The results were statistically analyzed by student’s t-test. The significant level established at 5% (P<0.05). The results of the methods of macrodilution, colony count and Agar well diffusion confirm the inhibitory effect of H-Av mixture on E.coli and E. fecalis. Statistically, there was no significant difference between the antibacterial effect of 2% chlorhexidine and 25% H-Av mixture (P>0.05) Increasing drug resistance to antimicrobial compounds needs to study of new drugs against pathogens. H-Av mixture with benefits such as availability, good taste and easier use than chlorhexidine, and fewer side effects can be a good option for intracanal irrigation after clinical trials.

Keywords


Antibacterial, Honey, Aloe vera extract, Enterococcus faecalis, Escherichia coli

Full Text:

PDF

References


Mohammadi Z. Antibiotics as intracanal medicaments: a review. J Calif Dent Assoc. 2009; 37(2):98-108.

Murray PE, Farber RM, Namerow KN, Kuttler S, Garcia-Godoy F. Evaluation of Morindacitrifolia as an endodontic irrigant. J Endod. 2008; 34(1):66-70.

Haapasalo M, Shen Y, Qian W, Gao Y. Irrigation in endodontics. Dent Clin North Am. 2010; 54(2):291-312.

Baby Joseph, S Justin Raj. A comparative study on various properties of five medicinally important plants. Int J of Pharmacology. 2011; 7(2):206-211.

Svitina H, Swanepoel R, Rossouw J, Netshimbupfe H, Gouws C, Hamman J. Treatment of Skin Disorders with Aloe Materials. Curr Pharm Des. 2019;25(20):2208-2240

Dong X, Zeng Y, Liu Y, You L, Yin X, Fu J, et al. Aloe-emodin: A review of its pharmacology, toxicity, and pharmacokinetics Phytother Res. 2020;34(2):270-281.

Jain S, Rathod N, Nagi R, Sur J, Laheji A, Gupta N, et al. Antibacterial Effect of Aloe vera gel against Oral Pathogens: An In-vitro Study J Clin Diagn Res. 2016; 10(11):ZC41-ZC44.

Gupta R, Thakur B, Singh P, Singh HB, Sharma VD, Katoch VM, et al. Anti-tuberculosis activity of selected medicinal plants against multi-drugresistant Mycobacterium tuberculosis isolates. Indian J Med Res. 2010; 131:809-813.

M. D. Mandal and S. Mandal, Honey: its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine. 2011;1(2): 154–160.

Boudreau MD, Beland FA. An evaluation of the biological and toxicological properties of Aloe Barbadensis (Miller), Aloe vera (Miller), Aloe vera. J Environ Sci Health C. 2006; 24:103–154.

Basrani B, Tjaderhane L, Snatos JM, Pascon E, Grad H, Lawrence HP, et al. Efficacy of chlorhexidine and calcium hydroxide – containing medicaments against E.faecalis invitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96: 618-624.

Popovska L, Zabokova Bilbilova E, Mircheva E, Stojanovska V. Chronic periapical lesions: correlations with clinical finding. Pril (Makedon Akad Nauk Umet Odd Med Nauki). 2014;35(1):263-269.

Agarry OO, Olaleye MT, Bello-Michael CO. Comparative antimicrobial activities of aloe veragel and leaf. African Journal of Biotechnology. 2005; 4(12):1413-1414.

Sureshchandra B, Kumar AJ. Antibacterial efficacy of Aloe veraextract on resistant antimicrobial strains in endodontics. Endodontology. 2011; 23(1):6-9.

George D, Bhat SS, Antony B. Comparative evaluation of the antimicrobial efficacy of Aloe veratooth gel and two popular commercial toothpastes: an in vitro study. Gen Dent. 2009; 57:238-241.

Wynn RL. Aloe vera gel: update for dentistry Gen Dent. 2005;53:6-9.

Ehsani M, Marashi M, Zabihi E, Issazadeh M, Khafri S. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study). Int J Mol Cell Med. 2013; 2(3):110-117.

French VM, Cooper RA, Molan PC. The antibacterial activity of honey against coagulase negative staphylococci. Antimicrob Chemother. 2005; 56(1): 228-231.

Ghori I, Ahmad SS. Antibacterial activities of honey, sandal oil and black pepper. Pak J Bot. 2009; 41: 461-466.

Wilkinson JM, Cavanagh HMA. Antibacterial Activity of 13 Honeys against Escherichia coli and Pseudomonas aeruginosa. J Med Food. 2005; 8(1): 100-103.

Adeleke OE, Olaitan JO, Okpekpe EI. Comparative antibacterial activity of honey and gentamicin against Escherichia coli and Pseudomonas aeruginosa. Annals of Burns and Fire Disasters. 2006 ; 19( 4):1-4.

Mercan N, Guvensen A, Celik A, Katircioglu H. Antimicrobial activity and pollen composition of honey samples collected from different provinces in Turkey. Nat Prod Res. 2007; 21(3): 187-195.

Tan HT, Rahman RA, Gan SH, Halim AS, Hassan SA, Sulaiman SA, et al. The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complementary and Alternative Medicine. 2009; 9: 34.

Goud S, Aravelli S, Dronamraju S, Cherukuri G, Morishetty P. Comparative Evaluation of the Antibacterial Efficacy of Aloe Vera, 3% Sodium Hypochlorite, and 2% Chlorhexidine Gluconate Against Enterococcus faecalis: An In Vitro Study. Cureus. 2018;10(10):3480.

Vasudeva A, Sinha DJ, Tyagi SP, Singh NN, Garg P, Upadhyay D. Disinfection of dentinal tubules with 2% Chlorhexidine gel, Calcium hydroxide and herbal intracanal medicaments against Enterococcus faecalis: An in-vitro study. Singapore Dent J. 2017;38:39-44.

Ehsani M, Amin Marashi M, Zabihi E, Issazadeh M, Khafri S. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study). Int J Mol Cell Med. 2013;2(3):110-116.

Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis. 2016; 6(2):71-79.




DOI: http://dx.doi.org/10.7575/aiac.abcmed.v.9n.1p.4

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

2013-2021 (CC-BY) Australian International Academic Centre PTY.LTD.

Advances in Bioscience and Clinical Medicine