Evaluation of the Relationship between Salivary Resistin and pH in Healthy Individuals

Solmaz Pourzare Mehrbani, Paria Motahari


Changes in composition and flow rate of saliva depict secondary systematic variations related to diseases, drugs and treatment of illnesses. This research examines the relationship between the saliva pH and saliva resistin in the seemingly healthy individuals. In this study 82 patients referring to the Department of Oral Medicine, Faculty of Tabriz Dentistry were examined. Before sampling from the patients with inclusive conditions, their mouths were washed by water and after 15 minutes 5ml of their saliva were collected by drooling in the sterile polyethylene containers. The collected samples were transferred to the laboratory and their pH was measured. Then the enzyme-linked immunosorbent assay (ELISA) test was performed by specific kit for saliva resistin measuring. The mean value of saliva resistin was 15.60±7.56 and pH mean value was 7.61±0.68. Results showed that about 61% of patients had resistin higher than15 ng/ml. According to the analysis results, 15.9% of patients had pH less than 7 while 57.3% had pH between 7-8 and 26.8% had pH higher than 8. There was a positive relationship between saliva resistin and pH. Since salivary resistin levels vary in response to different clinical conditions, direct relationship between the saliva resistin and pH indicates that pH changes can depict risk factor for specific diseases.


Resistin, pH, Saliva

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Heller R A, Schena M, Chai A, Shalon D, Bedilion T, Gilmore J, et al. Discovery and analysis of inflammatory disease-related genes using cDNA microarrays. Proceedings of the National Academy of Sciences. 1997;94(6):2150-2155.

Tremblay M, Loucif Y, Methot Jl, Brisson D, Gaudet D. Salivary pH as a marker of plasma adiponectin concentrations in women.Diabetol Metab Syndr. 2012; 4(4):34-42.

Mamali I,Roupas ND, Armeni AK, Theodoropoulou A, Markou KB, et al. Measurement of salivary resistin, visfatin and adiponectin levels. Peptides. 2012; 33(1): 120-124.

Gerber M, Boettner A, Seidel B, Lammert A, Bar J, Schuster E, et al. Serum resistin levels of obese and lean children and adolescents: biochemical analysis and clinical relevance. The Journal of Clinical Endocrinology & Metabolism. 2005; 90(8):4503-4509.

Azuma K, Katsukawa F, Oguchi S, Murata M, Yamazaki H, Shimada A, et al. Correlation between serum resistin level and adiposity in obese individuals. Obesity Research. 2003; 11(8): 997-1001.

Courten BV, Degawa-Yamauchi M, Considine RV, Tataranni PA. High serum resistin is associated with an increase in adiposity but not a worsening of insulin resistance in Pima Indians. Diabetes. 2004; 53(5):1279-1284.

Lee JH, Chan JL, Yiannakouris N, Kontogianni M, Estrada E, Seip R, et al. Circulating resistin levels are not associated with obesity or insulin resistance in humans and are not regulated by fasting or leptin administration: cross-sectional and interventional studies in normal, insulin-resistant, and diabetic subjects. J Clin Endocrinol Metab. 2003 ; 88(10):4848-56.

Owecki M, Nikisch E, Miczke A, Pupek-Musialik D, Sowiński J. Serum resistin is related to plasma HDL cholesterol and inversely correlated with LDL cholesterol in diabetic and obese humans. Neuro endocrinology letters. 2009; 31(5):673-678.

Kho HS, Lee SW, Chung SC, Kim YK. Oral manifestations and salivary flow rate, pH, and buffer capacity in patients with end-stage renal disease undergoing hemodialysis. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 1999; 88(3): 316-319.

Dawes C, Macpherson LMD. Effects of nine different chewing-gums and lozenges on salivary flow rate and pH. Caries research. 1992; 26(3): 176-182.

Parvinen T, Larmas M. The relation of stimulated salivary flow rate and pH to lactobacillus and yeast concentrations in saliva. Journal of dental research. 1981; 60(12): 1929-1935.

Kiess W, Meidert A, Dressendorfer RA, Schriever K, Kessler U, Konig A, et al. Salivary cortisol levels throughout childhood and adolescence: relation with age, pubertal stage, and weight.Pediatric Research. 1995; 37(4):502-506.

Tremblay M, Loucif Y, Methot J, Brisson D, Gaudet D. Salivary pH as a marker of plasma adiponectin concentrations in women.Diabetol Metab Syndr. 2012; 4(4):215-21.

Navazesh M. Methods for collecting saliva. Annals of the New York Academy of Sciences. 1993; 694:72-7.

Thorstensson H, Falk H, Hugosson A, Olsson J. Some salivary factors in Insulin dependent diabetics. Acta Odontal Scand. 1989; 43(3): 175-83.

Polland KE, Higgins F, Orchardson R. Salivary flow rate and PH during prolonged gum chewing in humans. J Oral Rehabilitation. 2003; 30(9): 861-65.

Greenberg M, Glick M. Burket΄s oral medicine diagnosis & treatment. 10th ed. Hamilton. BC Decker lnc. 2003. 563-77.

Aardal-Eriksson E, Karlberg BE, Holm AC. Salivary cortisol--an alternative to serum cortisol determinations in dynamic function tests. ClinChem Lab Med. 1998;36(4):215-22.

Venkatapathy R, Govindarajan V, Oza N, Parameswaran S, PennagaramDhanasekaran B, Prashad KV. Salivary creatinine estimation as an alternative to serum Creatinine in chronic kidney disease patients.Int J Nephrol. 2014;2014:742724.

Kaufman E, Lamster IB. Analysis of saliva for periodontal diagnosis--a review. J ClinPeriodontol. 2000 ;27(7):453-65.

Ozmeric N. Advances in periodontal disease markers. ClinChimActa. 2004;343(1-2):1-16.

Bárány E, Bergdahl IA, Bratteby LE, Lundh T, Samuelson G, Schütz A, Skerfving S, Oskarsson A. Trace element levels in whole blood and serum from Swedish adolescents. Sci Total Environ. 2002;286(1-3):129-41.

Pellegrini GG, Gonzales CM, Somoza JC, Friedman SM, Zeni SN. Correlation between salivary and serum markers of bone turnover in osteopenic rats. J eriodontol. 2008;79(1):158-65.

Baliga SH, S Muglikar S, Kale R, Salivary pH: A diagnostic biomarker, J Indian Soc Periodontol. 2013; 17(4): 461–465.

Al-Taee AF, khudhur AS. Determination of Salivary pH in Patients With Recurrent ApHthous Ulcera-tion (RAU). Al–Rafidain Dent J. 2010; 10(2):390-393.

Pokupec JS, Lukenda DB. Comorbidity of recurrent apHthous stomatitis and polyps ventriculi. Coll Antropol 2013;37(1):297-9.

Bakhshi M, Manifar S Tabatabaei F.S, Joz-khaje nori B, Sabour S, Rezaei Dokht F, Comparison of Salivary Biochemical Composition between End Stage Renal Disease and Healthy Subjects, J Mash Dent Sch. 2013; 37(3): 205-14.

Kuriakose S, Sundaresan C, Mathai V, Khosla E, Gaffoor FM. A comparative study of salivary buffering capacity, flow rate, resting pH, and salivary Immunoglobulin A in children with rampant caries and caries-resistant children. J Indian Soc Pedod Prev Dent. 2013;31(2):69-73.

Mittal M, Hassan B, Desai K, Duseja SH, S, Reddy SH.G, GCF Resistin As A Novel Marker in Patients with Chronic Periodontitis and Rheumatoid Arthritis, J Clin Diagn Res. 2015; 9(4): ZC62–ZC64.

Sabir DA, Ahmed M A. An Assessment of Salivary Leptin and Resistin Levels in Type Two Diabetic Patients with Chronic Periodontitis (A Comparative Study). Journal of Baghdad College of Dentistry. 2015; 27(4): 107-114.

Yin J, Gao H, Yang J, Xu L, Li M. Measurement of salivary resistin level in patients with type 2 diabetes. International journal of endocrinology. 2012; 12(4):125-129.

Karam TA, Al-Safi KA. An Evaluation of Serum and Salivary Adipokines (Leptin and Resistin) Levels in Periodontal Health and Disease.Journal of Baghdad College of Dentistry. 2015; 27(4): 119-124.

Thanakun S, Watanabe H, Thaweboon S, Izumi Y. An effective technique for the processing of saliva for the analysis of leptin and adiponectin. Peptides. 2013; 47:60-65.

DOI: http://dx.doi.org/10.7575/aiac.abcmed.v.8n.3p.12


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